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簡(jiǎn)要描述:CRL-1993 HMy2.CIR 人B淋巴母細(xì)胞CRL-1993 人B淋巴母細(xì)胞ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|:細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!
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HMy2.CIR 人B淋巴母細(xì)胞
IMDM培養(yǎng)基(GIBCO)+10%胎牛血清 細(xì)胞貨期8-10個(gè)工作日
上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師! 手機(jī)
培養(yǎng)基 The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
傳代方法 Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 viable cells/mL.
Interval: Maintain between 2 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
生長(zhǎng)條件 Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
生長(zhǎng)特性 suspension
存儲(chǔ)條件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium supplemented with 5% (v
) DMSO
Storage temperature: liquid nitrogen vapor phase
HMy2.CIR 人B淋巴母細(xì)胞
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